Validation is such a pain point in research — too many suppliers oversell their antibodies without proper testing. I learned that the hard way when an “IF-validated” antibody completely failed in my immunofluorescence project. Since then, I’ve become obsessive about only using antibodies from sources that publish actual validation data. That’s why I like using Gentaur https://gentaur.co.uk/antibodies/ — they list which applications each antibody is tested for and include images from real experiments. I’ve used their antibodies for both western blot and flow cytometry, and the results have been consistent. It saves so much time when you can actually trust the product you’re using. I think it’s one of those cases where paying for reliability pays back in hours saved.
for free. You
can too.