Creative Bioarray DDA Platform - Drug Discovery Accelerator Kinase Screening and Profiling Boost your kinase research with the most comprehensive set of assay techniques INTRODUCTION Kinases are arguably one of the most important drug targets due to the roles their dysregulation, mutations, and overexpression play in all major pathologies, including metabolic, cardiovascular, degenerative, inflammatory, and infectious diseases. Finding the best method for kinase screening and profiling can be a challenging task and will depend on your research objective, the type of kinase, availability of an antibody, the size of the kinase substrate and the analytical instruments available. Creative Bioarray provides a comprehensive set of assay technologies to solve all of your research objectives - from target discovery to compound screening, profiling and hit confirmation. Our breadth of expertise and experience in kinase research enables our scientists to support you every step of the way through the drug discovery process. KINASE ASSAYS There are a large number of approaches to evaluate kinase function and activity. In recent years, several different types of kinase assay technology have been developed, each of which have their relative merits and drawbacks. RADIOMETRIC ASSAY Radiometric kinase assay using 32P-labelled ATP or 33P-labelled ATP is one of the oldest techniques used to study protein phosphorylation. They rely on the transfer of radioactive phosphate groups from ATP to the substrate by a kinase, allowing detection of activity. The radiolabeled phosphate incorporated into the substrate is directly proportional to the kinase activity. Radiometric assay formats are the gold standard for kinase screening, which can produce reproducible, high-quality data and directly measure enzyme activity. But they are labor intensive and require safe disposal of radioactive materials, so they are usually not suitable for high-throughput screening.