A presentation at DevOpsDays Chicago in August 2019 in Chicago, IL, USA by Karissa Peth
Wow…. I miss scientific documentation
How is cancer research related to tech documentation?
Wow…. I miss scientific documentation
Determine the receptor(s) for the VISTA protein
Determine if VISTA protein binds certain types of cells Interrogate the differences between those cell types Run experiments to narrow down short list Run experiments to confirm receptor identity File patent & submit manuscript Screen develop anti-VISTA-R antibodies Confirm efficacy in cancer treatment
a Data what?
Data Sheet = Vendor Docs
Add density gradient medium to the tube by carefully pipetting it through the central hole. 2. Dilute sample with an equal volume of PBS + 2% FBS. Mix gently. 3. Add the diluted sample by pipetting it down the side of the tube. 4. Centrifuge at 1200 g for 10 minutes at room temperature with brake on. 5. Pour off the top layer, which contains the enriched MNCs, into a new tube. 6. Wash enriched MNCs with PBS + 2% FBS. Repeat wash.
Great documentation shifts your concentration from how to why
MATERIALS PBMC cultivation • RPMI 1640 medium • 100´ L-Glutamine stock solution (200 mM) • Human AB Serum • 24-well plate (e.g. Gas-permeable Culture Plate, # 150-000-362) ▲ Note: With the Gas-permeable Culture Plate (# 150-000-362), up to 2.5´107 PBMCs/well/mL can be stimulated as opposed to 1´107 PBMCs/well/mL in standard 24-well plates.
PREREQUISITES The following prerequisites are required for a successful and properly secured use of Helm. 1. A Kubernetes cluster 2. Deciding what security configurations to apply to your installation, if any 3. Installing and configuring Helm and Tiller, the cluster-side service.
Material Lists are the homework assignments for your docs.
Diagrams increase the accessibility of your docs.
Dates are a hint to users to expect differences between docs and real life.
Positive controls tell users they are heading the right way.
Rationale
Introduction Due to the very low frequency of ag-specific T cells in peripheral blood the reliable detection, enumeration and phenotypical characterization require the processing of high cell numbers and highly specific analysis methods. …. Combining magnetic cell enrichment and multiparameter flow cytometry analysis of CD154+ CD4+ T cells allow direct ex vivo detection and characterization of rare ag-specific T cells.
When to use them Heatmaps look best when you have a lot of events to visualize, and where the spread of values is wide enough to see some differentiation, but not complete noise. Any column representing a duration or size is a perfect fit, but any column you might run a percentile or average calculation on may benefit from being rendered as a heatmap as well.
Rationale in your docs creates lasting impact.
Wow…. I miss scientific documentation
Materials List Diagrams Dates Positive Controls Rationale/Uses
Great documentation shifts your concentration from how to why
https://giphy.com/gifs/computer-gif-artist-code-fedryX7dMGMe6lgqDm https://www.mskcc.org/car-cell-therapy https://www.mibioresearch.com/services/flow-cytometry/ https://stackoverflow.blog/2010/12/17/introducing-programmers-stackexchange-com/ http://www.agileo365.com/2019/03/Tracking-Agile-Progress-The-Sprint-Backlog.html https://blogs.technet.microsoft.com/yuridiogenes/2018/05/03/leveraging-azure-security-center-capabilities-in-a- pcidss-compliant-environment/ https://giphy.com/gifs/funny-flashback-12UtDavMK3xHmo https://twitter.com/ThePracticalDev/status/705825638851149824 https://dev.to/rly https://cdn.stemcell.com/media/files/pis/DX20655-PIS_1_1_0.pdf?_ga=2.213533332.1481748328.15666712161695496037.1566341450 https://www.miltenyibiotec.com/_Resources/Persistent/49ab790c21d8e328d07679dc66205cba48357cd7/SP_Rapid_ARTE.pdf https://helm.sh/docs/using_helm/#prerequisites https://learn.hashicorp.com/terraform?track=azure#azure https://docs.microsoft.com/en-us/azure/functions/tutorial-static-website-serverless-api-with-database-old https://www.miltenyibiotec.com/US-en/resources/macs-handbook/human-cells-and-organs/human-cell-types/cd4-t-cellshuman.html https://docs.microsoft.com/en-us/azure/aks/virtual-nodes-cli?view=azure-cli-latest https://docs.honeycomb.io/working-with-your-data/heatmaps/#when-to-use-them https://docs.microsoft.com/en-us/azure/functions/tutorial-static-website-serverless-api-with-database-old?tutorialstep=1 https://commons.wikimedia.org/wiki/File:PC3_prostate_cancer_cells,_confocal_image,_63x.jpg https://blogs.biomedcentral.com/on-medicine/2016/05/27/winners-breast-cancer-research-image-competition-2016/
